Details

Autor(en) / Beteiligte

• Petitpas, F.
• Sichel, F.
• Hébert, B.
• Lagadu, S.
• Beljean, M.
• Pottier, D.
• Laurentie, M.
• Prevost, V.

Titel

Effects of alcohol consumption on biomarkers of oxidative damage to DNA and lipids in ethanol-fed pigs

Ist Teil von

• Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2013-03, Vol.65 (3), p.263-269

Ort / Verlag

Germany: Elsevier GmbH

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Chronic alcohol consumption is known to result in tissue injury, particularly in the liver, and is considered a major risk factor for cancers of the upper respiratory tract. Here we assessed the oxidative effects of subchronic ethanol consumption on DNA and lipids by measuring biomarkers 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and malondialdehyde (MDA), respectively. Physiological responses of pigs (n=4) administered ethanol in drinking water for 39 days were compared with those of water-fed pigs (n=4). Alcoholisation resulted in serum ethanol concentration of 1.90gL−1 and in a moderate but significant increase in alanine aminotransferase activity, an index of liver injury. However, between the alcoholised and control groups there were no significant differences in the levels of 8-oxodG (8-oxodG per 106 2′deoxyguanosine) from leucocytes (2.52±0.42 Vs 2.39±0.34) or from target organs, liver, cardia and oesophagus. Serum MDA levels were also similar in ethanol-fed pigs (0.33±0.04μM) and controls (0.28±0.03μM). Interestingly, levels of 8-oxodG in cardia were positively correlated with those in oesophagus (Spearman correlation coefficient R=1, P<0.0001). Our results suggest that alcohol consumption may not cause oxidative damage to DNA and lipids as measured by 8-oxodG and MDA, respectively. The duration of alcoholisation and the potential alcohol-induced nutritional deficiency may be critical determinants of ethanol toxicity. Relevant biomarkers, such as factors involved in sensitization to ethanol-induced oxidative stress are required to better elucidate the relationship between alcohol consumption, oxidative stress and carcinogenesis.