Nature communications, 2015-01, Vol.6 (1), p.6174-6174, Article 6174
2015
Details
- Autor(en) / Beteiligte
- Titel
- Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence
- Ist Teil von
- Nature communications, 2015-01, Vol.6 (1), p.6174-6174, Article 6174
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Mast cells are tissue-resident immune cells that play a key role in inflammation and allergy. Here we show that interaction of mast cells with antibody-targeted cells induces the polarized exocytosis of their granules resulting in a sustained exposure of effector enzymes, such as tryptase and chymase, at the cell-cell contact site. This previously unidentified mast cell effector mechanism, which we name the antibody-dependent degranulatory synapse (ADDS), is triggered by both IgE- and IgG-targeted cells. ADDSs take place within an area of cortical actin cytoskeleton clearance in the absence of microtubule organizing centre and Golgi apparatus repositioning towards the stimulating cell. Remarkably, IgG-mediated degranulatory synapses also occur upon contact with opsonized Toxoplasma gondii tachyzoites resulting in tryptase-dependent parasite death. Our results broaden current views of mast cell degranulation by revealing that human mast cells form degranulatory synapses with antibody-targeted cells and pathogens for dedicated secretion and defence.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/ncomms7174Titel-ID: cdi_hal_primary_oai_HAL_hal_03533120v1
- Format
- –
- Schlagworte
- Actins - metabolism, Adaptor Proteins, Signal Transducing - metabolism, Antibodies, Monoclonal - pharmacology, B-Lymphocytes - drug effects, B-Lymphocytes - immunology, Cell Death - drug effects, Cell Degranulation - drug effects, Cell Degranulation - immunology, Cell Membrane - drug effects, Cell Membrane - metabolism, Cell Polarity - drug effects, Chemokines - biosynthesis, Cytoplasmic Granules - drug effects, Cytoplasmic Granules - metabolism, Humans, Humans Immunoglobulin E / immunology, Immunoglobulin E - immunology, Immunological Synapses - drug effects, Immunological Synapses - metabolism, Immunology, Life Sciences, Ligands, Mast Cells - drug effects, Mast Cells - immunology, Mast Cells - physiology, Mast Cells - secretion, Mast Cells / metabolism, Membrane Proteins - metabolism, Molecular Dynamics Simulation, Opsonin Proteins - metabolism, Phosphorylation - drug effects, Receptors, IgE - metabolism, Rituximab - pharmacology, Toxicology, Toxoplasma - drug effects, Toxoplasma - physiology