Details

Autor(en) / Beteiligte

• Bridiau, Nicolas
• Taboubi, Selma
• Marzouki, Nejib
• Legoy, Marie Dominique
• Maugard, Thierry

Titel

β-Galactosidase Catalyzed Selective Galactosylation of Aromatic Compounds

Ist Teil von

• Biotechnology progress, 2006, Vol.22 (1), p.326-330

Ort / Verlag

USA: American Chemical Society

Erscheinungsjahr

2006

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• A new approach to galacto‐oligosaccharides and galacto‐conjugates synthesis performed by the β‐galactosidase from Kluyveromyces lactisis reported. The enzymatic galactosylation of eight kinds of adsorbed aromatic primary alcohols, in particular the two drugs guaifenesin and chlorphenesin, gave the corresponding β‐d‐galacto‐pyranosides in yields ranging between approximately 10% and 96%. For the first time, we have showed that the adsorption of acceptor substrates onto solid supports such as silica gel influences the yield and the selectivity of galacto‐conjugates synthesis. In particular, we observed that adsorption of acceptor favored the synthesis of digalactosylated compounds.