Details

Autor(en) / Beteiligte

• Pawlak, André
• Belbekhouche, Sabrina

Titel

New approach to develop functionalized polyelectrolyte tube using bacteria as template

Ist Teil von

• Journal of applied polymer science, 2022-02, Vol.139 (8), p.n/a

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Herein, we propose a new approach consisting of using bacteria as a starting point for the development of new material of interest. Indeed, we are interested in using bacteria as a template. We focus on Escherichia coli which are one of the best studied microorganisms. They are in the micrometer range size and present a tubular shape. We thus use E. coli cells as biotemplate for the design of polyelectrolyte tubes. Indeed, E. coli presents a negatively charged surface (−17 mV) which is then coated by charged polymer via the layer‐by‐layer process. We use chitosan as a polycation and a dextran sulfate as polyanion. First, we evidence the controlled coating of the surface of the bacteria via the measurement of zeta potential (+25 mV to −20 mV) and also by fluorescent microscopy using a fluorescently tagged polysaccharide (fluorescein isothiocyanate‐dextran). Secondly, we remove the bacteria core by using sodium hypochlorite and thus we obtain hollow polyelectrolyte tubes (dimensions of 1.1–1.5 μm × 2.0–6.0 μm). Such bioinspired materials are of major interest for a wide range of applications for the fabrication of polymeric tubes. We propose a new approach consisting in using bacteria as a starting point for the development of new material of interest. Indeed, bacteria has been coated by charged natural polymers (polyelectrolytes) via the layer‐by‐layer process and finally been removed to obtain hollow polyelectrolytes tubes