Details

Autor(en) / Beteiligte

• Moulis, Claire
• Guieysse, David
• Morel, Sandrine
• Séverac, Etienne
• Remaud-Siméon, Magali

Titel

Natural and engineered transglycosylases: Green tools for the enzyme-based synthesis of glycoproducts

Ist Teil von

• Current opinion in chemical biology, 2021-04, Vol.61, p.96-106

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• An increasing number of transglycosylase-based processes provide access to oligosaccharides or glycoconjugates, some of them reaching performance levels compatible with industrial developments. Nevertheless, the full potential of transglycosylases has not been explored because of the challenges in transforming a glycoside hydrolase into an efficient transglycosylase. Advances in studying enzyme structure/function relationships, screening enzyme activity, and generating synthetic libraries guided by computational protein design or machine learning methods should considerably accelerate the development of these catalysts. The time has now come for researchers to uncover their possibilities and learn how to design and precisely refine their activity to respond more rapidly to the growing demand for well-defined glycosidic structures.