Details

Autor(en) / Beteiligte

• Bates, G.P
• Valdes, J
• Hummerich, H
• Baxendale, S
• Le Paslier, D.L
• Monaco, A.P
• Tagle, D
• MacDonald, M.E
• Altherr, M
• Ross, M

Titel

Characterization of a yeast artificial chromosome contig spanning the Huntington's disease gene candidate region

Ist Teil von

• Nature genetics, 1992-06, Vol.1 (3), p.180-187

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

1992

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The Huntington's disease (HD) gene has been localized by recombination events to a region covering 2.2 megabases (Mb) DNA within chromosome 4p16.3. We have screened three yeast artificial chromosome (YAC) libraries in order to isolate and characterize 44 YAC clones mapping to this region. Approximately 50% of the YACs were chimaeric. Unstable YACs were identified across the whole region, but were particularly prevalent around the D4S183 and D4S43 loci. The YACs have been assembled into a contig extending from D4S126 to D4S98 covering roughly 2 Mb DNA, except for a gap of about 250 kilobases (kb). The establishment of a YAC contig which spans the region most likely to contain the HD mutation is an essential step in the isolation of the HD gene.