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SUMMARY Field studies have characterized natural faults as rough, non-planar surfaces at all scales. Fault roughness induces local stress perturbations during slip, which dramatically affect rupture behaviour, resulting in slip heterogeneity. However, the relation between fault roughness and slip heterogeneity remains a key knowledge gap between current numerical and field studies. In this study, we analyse numerical simulations of earthquake rupture to determine how roughness influences final slip. Using a rupture catalogue containing thousands of dynamic rupture simulations on band-limited self-similar fractal fault profiles with varying roughness and background shear stress levels, we quantify how fault roughness affects the spectral characteristics of the resulting slip distribution. We find that slip distributions become increasingly more self-affine, that is, containing more short wavelength fluctuations as compared to the self-similar fault profiles, as roughness increases. We also find that, at very short wavelengths (<1 km), the fractal dimension of the slip distributions dramatically changes with increasing roughness, background shear stress, and rupture speed (sub-Rayleigh versus supershear). The existence of a critical wavelength around 1 km, under which more short wavelengths are either preserved or created, suggests the role of rupture process and dynamic effects, together with fault geometry, in controlling the final slip distributions. The same spectral analysis is performed on high-resolution coseismic surface slip distributions from a catalogue of real strike-slip earthquakes. Compared to numerical simulations, all earthquakes feature slip distributions that are much more self-affine than the slip distributions from numerical simulations. A different critical wavelength, here around 5–6 km, appears, potentially informing about a critical asperity length. While we show here that the relation between fault roughness and slip is much more complex than expected, this study is a first attempt at using statistical analyses of numerical simulations on rough faults to investigate observed coseismic slip distributions.