Details

Autor(en) / Beteiligte

• Gálvez, Eric J.C.
• Iljazovic, Aida
• Amend, Lena
• Lesker, Till Robin
• Renault, Thibaud
• Thiemann, Sophie
• Hao, Lianxu
• Roy, Urmi
• Gronow, Achim
• Charpentier, Emmanuelle
• Strowig, Till

Titel

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp

Ist Teil von

• Cell host & microbe, 2020-12, Vol.28 (6), p.838-852.e6

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance. [Display omitted] •Metagenomics and culturomics characterization of Prevotella spp. in the mouse gut•Distinct Prevotella spp. compete in vivo for similar plant-derived polysaccharides•A dominant Prevotella strain encodes a specific PUL enabling arabinoxylan utilization•Similar genetic features are found in human Prevotella copri strains enriched in vegans Prevotella are among the most dominant bacteria found in the gut. Galvez et al. identify Prevotella species in the mouse gut and examine how one dominant species outcompetes commensal bacteria, including other Prevotella species, by encoding specific polysaccharide utilization loci (PULs), notably those enabling arabinoxylan utilization.