Details

Autor(en) / Beteiligte

• Bozsoki, Zoltan
• Gysel, Kira
• Hansen, Simon B.
• Lironi, Damiano
• Krönauer, Christina
• Feng, Feng
• de Jong, Noor
• Vinther, Maria
• Kamble, Manoj
• Thygesen, Mikkel B.
• Engholm, Ebbe
• Kofoed, Christian
• Fort, Sébastien
• Sullivan, John T.
• Ronson, Clive W.
• Jensen, Knud J.
• Blaise, Mickaël
• Oldroyd, Giles
• Stougaard, Jens
• Andersen, Kasper R.
• Radutoiu, Simona

Titel

Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Ist Teil von

• Science (American Association for the Advancement of Science), 2020-08, Vol.369 (6504), p.663-670

Ort / Verlag

Washington: The American Association for the Advancement of Science

Erscheinungsjahr

2020

Quelle

Science magazine suite

Beschreibungen/Notizen

• Switching perception of friend and foe Lysine motif receptors in plants perceive glycans that signal the presence of pathogenic or symbiotic nitrogen-fixing microbes. Bozsoki et al. now define the portions of these receptors that create the discriminatory binding pocket (see the Perspective by Bisseling and Geurts). The motifs were conserved in receptors that initiate immune responses, reflecting the invariable nature of the chitin fragments that they sense. Conversely, the motifs in receptors that respond to symbiotic signals were more varied, reflecting the greater diversity of the lipochitooligosaccharides (Nod factors) that they sense. With domain swapping, the authors switched the Nod factor specificity of receptors from two legume species and also enabled a chitin receptor that was otherwise dedicated to the detection of pathogenic microbes to instead recognize Nod factors. Science , this issue p. 663 ; see also p. 620 Protein domain swaps into existing plant nodulation and immune response receptors may enable rhizobial symbiosis in nonlegumes. Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicus initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.