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Cloning and expression in yeast of a plant potassium ion transport system
Ist Teil von
Science (American Association for the Advancement of Science), 1992-05, Vol.256 (5057), p.663-665
Ort / Verlag
Washington, DC: American Society for the Advancement of Science
Erscheinungsjahr
1992
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or (86)Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region