FEMS microbiology letters, 1999-12, Vol.181 (1), p.145-152
1999
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Details
- Autor(en) / Beteiligte
- Titel
- xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria
- Ist Teil von
- FEMS microbiology letters, 1999-12, Vol.181 (1), p.145-152
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1999
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1097eISSN: 1574-6968DOI: 10.1111/j.1574-6968.1999.tb08837.xTitel-ID: cdi_hal_primary_oai_HAL_hal_02697977v1
- Format
- –
- Schlagworte
- active sites, Amino Acid Sequence, Anaerobiosis, Animals, Bacteria, Base Sequence, Biochemistry. Physiology. Immunology. Molecular biology, Biodegradation, Biological and medical sciences, cDNA libraries, Cell walls, Ciliophora - enzymology, Ciliophora - genetics, Cloning, complementary DNA, DNA, Complementary - genetics, Domains, Electrophoresis, Polyacrylamide Gel, Enzymes, Eukaryotes, eukaryotic cells, Fundamental and applied biological sciences. Psychology, Fungi, Glucans - metabolism, Glycoside hydrolase, glycosides, Gram-positive bacteria, Gram-Positive Bacteria - enzymology, Gram-Positive Bacteria - genetics, Hydrolase, Immunoblotting, Life Sciences, Microbiology, Microbiology and Parasitology, Molecular Sequence Data, Phylogeny, Polyplastron multivesiculatum, Polysaccharides, Protozoa, Rumen, Rumen - parasitology, Sequence Analysis, DNA, sequence homology, Xylan Endo-1,3-beta-Xylosidase, Xylanase, xylanases, Xylans - metabolism, Xylosidases - chemistry, Xylosidases - genetics, Xylosidases - metabolism