Details

Autor(en) / Beteiligte

• Guihot, Gwénaële
• Colomb, Virginie
• Jobert-Giraud, Agathe
• Morel, Marie-Thérèse
• Corriol, Odile
• Duée, Pierre-Henri
• Ricour, Claude
• Blachier, François

Titel

Effect of an Elemental vs a Complex Diet on Polyamine Metabolism in Rat Isolated Enterocytes

Ist Teil von

• JPEN. Journal of parenteral and enteral nutrition, 1997-09, Vol.21 (5), p.259-265

Ort / Verlag

Thousand Oaks, CA: Sage Publications

Erscheinungsjahr

1997

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Background: Polyamines play an important role in the proliferation and differentiation of enterocytes. Ornithine decarboxylase (ODC) is the rate-limiting enzyme for polyamine biosynthesis. Elemental diets, providing easily absorbable nutrients such as free amino acids, are used in clinical practice to treat growth failure and malnutrition. They are very different from complex diets normally consumed. Little information is available about the influence of elemental diets on metabolic capacities of enterocytes. This study was undertaken in rats to assess the effects on polyamine metabolism of an elemental diet compared with a complex diet. Methods: Rats were fed the elemental diet (group ED) or the control diet (group C) for 14 days. The dietary intakes were isocaloric and isonitrogenous in groups C and ED. Villous enterocytes were then isolated and metabolic capacities or enzyme activities were assessed. Results: Both the enterocyte capacity to decarboxylate ornithine through ODC (measured in viable enterocytes) and ODC activity (measured in homogenates) were severely decreased in group ED. The polyamine content in enterocytes, however, was maintained at a similar level in both groups. This coincided with a decrease in the main enzymatic activity responsible for putrescine catabolism (ie, diamine oxidase activity) in group ED. Conclusions: Although nutrition manipulation was shown to alter polyamine biosynthesis in this study, the polyamine homeostasis was probably maintained at least in part, through down-regulation of diamine oxidase. (Journal of Parenteral and Enteral Nutrition 21:259-265, 1997)