Details

Autor(en) / Beteiligte

• Daniel, Catherine
• Triboï, Eugène

Titel

Isolation of Wheat Grain Compartments and Their Protein Composition

Ist Teil von

• Cereal research communications, 2001-01, Vol.29 (1/2), p.197-204

Ort / Verlag

Cereal Research Non-Profit Company

Erscheinungsjahr

2001

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The structure and the composition of the wheat (Triticum aestivum L.) grain are heterogeneous. In order to study the composition of grain compartments, we developed a method to easily isolate a relative large amount of endosperms and embryos. The analysis of proteins were made as an example of compartimentation and heterogeneity of grain composition. Proteins were sequentially extracted and analysed by RP-HPLC. Gliadin and glutenin are specific to the endosperm, whereas some albumins-globulins are found in the endosperm, some in the embryo and others are common in both sites. The quantitative contribution of embryo proteins to the whole grain proteins however was not significant because of the low proportion of embryo weight to whole grain dry matter. Thus for proteins, the analyses of flour could be considered as equivalent as the analysis of the whole grain.