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The button mushroom, Agaricus bisporus (Lange) Imbach, the most common cultivated mushroom, is susceptible to a wide range of virus, bacterial, and fungal diseases. However, only some diseases were studied for the mechanisms involved in the host-microorganism interaction. This review deals with biochemical mechanisms related to cavity disease (Burkholderia gladioli) and to the interaction between A. bisporus and the causal agents responsible for the most severe diseases, namely the bacteria Pseudomonas tolaasii and Pseudomonas reactans and the fungi Trichoderma aggressivum and Lecanicillium fungicola.