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Microarray analysis of humic acid effects on Brassica napus growth: Involvement of N, C and S metabolisms
Ist Teil von
Plant and soil, 2012-10, Vol.359 (1/2), p.297-319
Ort / Verlag
Dordrecht: Springer
Erscheinungsjahr
2012
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
Background & aims Winter rapeseed (Brassica napus) is characterized by a low N recovery in seeds and requires high rates of fertilization to maintain yield. Its nutrient use efficiency could be improved by addition of a biostimulant such as humic acids whose physiological effects have been described previously in some plant species. However, to our knowledge, no study has focused on transcriptomic analyses to determine metabolic targets of this extract. Methods A preliminary screening of ten humic acids revealed a significant effect of one of them (HA7) on rapeseed root growth. Microarray analysis was then used on HA7-treated or non-treated plants to characterize changes in gene expression that were further supported by physiological evidence. Results Stimulation of nitrogen uptake (+15% in shoots and +108% in roots) and assimilation was found to be increased in a similar manner to growth while sulfate content (+76% in shoots and +137% in roots) was more strongly stimulated leading to higher sulfate accumulation. In parallel, microscopic analysis showed an enhancement of chloroplast number per cell. Conclusion It is therefore suggested that HA7, which promotes plant growth and nutrient uptake, could be used as a supplementary tool to improve rapeseed nitrogen use efficiency.