Details

Autor(en) / Beteiligte

• Rolhion, Nathalie
• Furniss, R. Christopher D.
• Grabe, Grzegorz
• Ryan, Aindrias
• Liu, Mei
• Matthews, Sophie A.
• Holden, David W.

Titel

Inhibition of Nuclear Transport of NF-kappa B p65 by the Salmonella Type III Secretion System Effector SpvD

Ist Teil von

• PLoS pathogens, 2016-05, Vol.12 (5)

Ort / Verlag

Public Library of Science

Erscheinungsjahr

2016

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Salmonella enterica replicates in macrophages through the action of effector proteins translocated across the vacuolar membrane by a type III secretion system (T3SS). Here we show that the SPI-2 T3SS effector SpvD suppresses proinflammatory immune responses. SpvD prevented activation of an NF-kappa B-dependent promoter and caused nuclear accumulation of importin-a, which is required for nuclear import of p65. SpvD interacted specifically with the exportin Xpo2, which mediates nuclear-cytoplasmic recycling of importins. We propose that interaction between SpvD and Xpo2 disrupts the normal recycling of importin-a from the nucleus, leading to a defect in nuclear translocation of p65 and inhibition of activation of NF-kappa B regulated promoters. SpvD down-regulated pro-inflammatory responses and contributed to systemic growth of bacteria in mice. This work shows that a bacterial pathogen can manipulate host cell immune responses by interfering with the nuclear transport machinery.