Plant physiology (Bethesda), 2019-05, Vol.180 (1), p.392-403
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Antagonistic Actions of FPA and IBM2 Regulate Transcript Processing from Genes Containing Heterochromatin
- Ist Teil von
- Plant physiology (Bethesda), 2019-05, Vol.180 (1), p.392-403
- Ort / Verlag
- United States: Oxford University Press, American Society of Plant Biologists
- Erscheinungsjahr
- 2019
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Repressive epigenetic marks, such as DNA and histone methylation, are sometimes located within introns. In Arabidopsis ( ), INCREASE IN BONSAI METHYLATION2 (IBM2), an RNA-binding protein containing a bromo-adjacent homology domain, is required to process functional transcript isoforms of genes carrying intronic heterochromatin. In a genetic screen for suppressors of the mutation, we identified FPA, an RNA-binding protein that promotes use of proximal polyadenylation sites in genes targeted by IBM2, including encoding an essential H3K9 histone demethylase and the disease resistance gene Both IBM2 and FPA are involved in the processing of their common mRNA targets: Transcription of IBM2 target genes is restored when is mutated in and impaired in transgenic plants overexpressing By contrast, transposons targeted by IBM2 and localized outside introns are not under this antagonistic control. The DNA methylation patterns of some genes and transposons are modified in plants, including the large intron of , but these changes are rather limited and reversed when the mutant is complemented, indicating that FPA has a restricted role in mediating silencing. These data reveal a complex regulation by IBM2 and FPA pathways in processing mRNAs of genes bearing heterochromatic marks.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.18.01106Titel-ID: cdi_hal_primary_oai_HAL_hal_02628340v1
- Format
- –
- Schlagworte
- Arabidopsis - genetics, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Chemical and Process Engineering, DNA Methylation, DNA Transposable Elements, Engineering Sciences, Food engineering, Gene Expression Regulation, Plant, Genes, Suppressor, Heterochromatin - genetics, Introns, Jumonji Domain-Containing Histone Demethylases - genetics, Jumonji Domain-Containing Histone Demethylases - metabolism, Life Sciences, Mutation, Plants, Genetically Modified, Polyadenylation, RNA, Messenger - genetics, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism, Vegetal Biology