Journal of industrial microbiology & biotechnology, 2009-12, Vol.36 (12), p.1529-1534
2009
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- Autor(en) / Beteiligte
- Titel
- Saccharomyces cerevisiae-based bioassay for assessing pesticide toxicity
- Ist Teil von
- Journal of industrial microbiology & biotechnology, 2009-12, Vol.36 (12), p.1529-1534
- Ort / Verlag
- Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag
- Erscheinungsjahr
- 2009
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- This study evaluates the toxic effect of three pesticides (Azoxystrobin, Cymoxanil, and Diuron) on the yeast Saccharomyces cerevisiae for the development of a new bioassay based on inhibition of S. cerevisiae metabolic activity at the level of adenosine-5-triphosphate (ATP) synthesis, as compared with two different toxicity tests based on inhibition of Daphnia magna mobility (NF EN ISO 6341) and inhibition of Vibrio fisheri activity (NF EN ISO 11348). The S. cerevisiae bioassay is cheaper and 96 times faster than the D. magna toxicity bioassay, but has lower sensitivity. It is as fast as the V. fisheri bioassay and more sensitive. Thus, this new toxicity test can be proposed for rapid detection of pesticide residues in environmental samples as a complement to the more expensive and time-consuming D. magna toxicity test.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1367-5435eISSN: 1476-5535DOI: 10.1007/s10295-009-0649-1Titel-ID: cdi_hal_primary_oai_HAL_hal_02594947v1
- Format
- –
- Schlagworte
- Adenosine, Adenosine Triphosphate - metabolism, Agricultural production, Aliivibrio fischeri - metabolism, Animals, Bioassays, Biochemistry, Bioethics, Bioinformatics, Biological and medical sciences, Biological Assay, Biomedical and Life Sciences, Biotechnology, Daphnia - metabolism, Daphnia magna, Diuron, Environmental impact, Environmental Pollutants - toxicity, Environmental Sciences, Freshwater, Fundamental and applied biological sciences. Psychology, Fungicides, Gene expression, Genetic Engineering, Inhibitory Concentration 50, Inorganic Chemistry, Life Sciences, Metabolism, Microbiology, Microorganisms, Pesticide residues, Pesticide toxicity, Pesticides, Pesticides - toxicity, Pollutants, Regression analysis, Respiration, Saccharomyces cerevisiae, Saccharomyces cerevisiae - drug effects, Saccharomyces cerevisiae - metabolism, Sensitivity and Specificity, Short Communication, Studies, Toxicity, Toxicity Tests - methods, Yeast, Yeasts