Details

Autor(en) / Beteiligte

• Giordani, Lorenzo
• He, Gary J.
• Negroni, Elisa
• Sakai, Hiroshi
• Law, Justin Y.C.
• Siu, M. Mona
• Wan, Raymond
• Corneau, Aurélien
• Tajbakhsh, Shahragim
• Cheung, Tom H.
• Le Grand, Fabien

Titel

High-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle-Resident Cell Populations

Ist Teil von

• Molecular cell, 2019-05, Vol.74 (3), p.609-621.e6

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Adult tissue repair and regeneration require stem-progenitor cells that can self-renew and generate differentiated progeny. Skeletal muscle regenerative capacity relies on muscle satellite cells (MuSCs) and their interplay with different cell types within the niche. However, our understanding of skeletal muscle tissue cellular composition is limited. Here, using a combined approach of single-cell RNA sequencing and mass cytometry, we precisely mapped 10 different mononuclear cell types in adult mouse muscle. We also characterized gene signatures and determined key discriminating markers of each cell type. We identified two previously understudied cell populations in the interstitial compartment. One expresses the transcription factor scleraxis and generated tenocytes in vitro. The second expresses markers of smooth muscle and mesenchymal cells (SMMCs) and, while distinct from MuSCs, exhibited myogenic potential and promoted MuSC engraftment following transplantation. The blueprint presented here yields crucial insights into muscle-resident cell-type identities and can be exploited to study muscle diseases. [Display omitted] •Combination of single-cell RNA sequencing and mass cytometry•Construction of a cell atlas of adult skeletal muscle•Skeletal muscle is composed of 10 mononucleated cell types and myofibers•Skeletal muscle contains interstitial tenocytes and smooth muscle-mesenchymal cells A strong characterization of cell types present in adult skeletal muscle is needed. Giordani et al. use both single-cell transcriptomics and mass cytometry to build a single-cell survey of adult skeletal muscle tissue and reveal understudied cell populations.