Details

Autor(en) / Beteiligte

• Keriel, Anne
• Botella, Eric
• Estrach, Soline
• Bragagnolo, Gabriel
• Vergunst, Annette C.
• Feral, Chloe C.
• O'Callaghan, David

Titel

Brucella Intracellular Life Relies on the Transmembrane Protein CD98 Heavy Chain

Ist Teil von

• The Journal of infectious diseases, 2015-06, Vol.211 (11), p.1769-1778

Ort / Verlag

United States: Oxford University Press

Erscheinungsjahr

2015

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Brucella are intracellular bacterial pathogens that use a type IV secretion system (T4SS) to escape host defenses and create a niche in which they can multiply. Although the importance of Brucella T4SS is dear, little is known about its interactions with host cell structures. In this study, we identified the eukaryotic protein CD98hc as a partner for Brucella T4SS subunit VirB2. This transmembrane glycoprotein is involved in amino acid transport, modulation of integrin signaling, and cell-to-cell fusion. Knockdown of CD98hc expression in HeLa cells demonstrated that it is essential for Brucella infection. Using knockout dermal fibroblasts, we confirmed its role for Brucella but found that it is not required for Salmonella infection. CD98hc transiently accumulates around the bacteria during the early phases of infection and is required for both optimal bacterial uptake and intracellular multiplication of Brucella. These results provide new insights into the complex interplay between Brucella and its host.