Details

Autor(en) / Beteiligte

• Salvatore, Giulia
• Bernoud-Hubac, Nathalie
• Bissay, Nathalie
• Debard, Cyrille
• Daira, Patricia
• Meugnier, Emmanuelle
• Proamer, Fabienne
• Hanau, Daniel
• Vidal, Hubert
• Aricò, Maurizio
• Delprat, Christine
• Mahtouk, Karène

Titel

Human monocyte-derived dendritic cells turn into foamy dendritic cells with IL-17A

Ist Teil von

• Journal of lipid research, 2015-06, Vol.56 (6), p.1110-1122

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Interleukin 17A (IL-17A) is a proinflammatory cytokine involved in the pathogenesis of chronic inflammatory diseases. In the field of immunometabolism, we have studied the impact of IL-17A on the lipid metabolism of human in vitro-generated monocyte-derived dendritic cells (DCs). Microarrays and lipidomic analysis revealed an intense remodeling of lipid metabolism induced by IL-17A in DCs. IL-17A increased 2-12 times the amounts of phospholipids, cholesterol, triglycerides, and cholesteryl esters in DCs. Palmitic (16:0), stearic (18:0), and oleic (18:ln-9c) acid were the main fatty acid chains present in DCs. They were strongly increased in response to IL-17A while their relative proportion remained unchanged. Capture of extracellular lipids was the major mechanism of lipid droplet accumulation, visualized by electron microscopy and Oil Red O staining. Besides this foamy phenotype, IL-17A induced a mixed macrophage-DC phenotype and expression of the nuclear receptor NR1H3/liver X receptor-α, previously identified in the context of atherosclerosis as the master regulator of cholesterol homeostasis in macrophages. These IL-17A-treated DCs were as competent as untreated DCs to stimulate allogeneic naive T-cell proliferation. Following this first characterization of lipid-rich DCs, we propose to call these IL-17A-dependent cells "foamy DCs" and discuss the possible existence of foamy DCs in atherosclerosis, a metabolic and inflammatory disorder involving IL-17A.