Details

Autor(en) / Beteiligte

• Kazane, Imen
• Gorgy, Karine
• Gondran, Chantal
• Spinelli, Nicolas
• Zazoua, Ali
• Defrancq, E
• Cosnier, Serge

Titel

Highly Sensitive Bisphenol‑A Electrochemical Aptasensor Based on Poly(Pyrrole-Nitrilotriacetic Acid)-Aptamer Film

Ist Teil von

• Analytical chemistry (Washington), 2016-07, Vol.88 (14), p.7268-7273

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2016

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• An electrochemical highly sensitive aptasensor was developed based on electropolymerized poly­(pyrrole-nitrilotriacetic) acid film and a new aptamer functionalized by a pentahistidine peptide for the quantification of bisphenol A. A surface coverage of antibisphenol A aptamer of 1.84 × 10–10 mol cm–2 was estimated from the electrochemical signal of the [RuIII(NH3)6]3+ complex bound by electrostatic interactions onto the aptamer-modified electrode. The binding of bisphenol A onto the polymer film was successfully characterized by electrochemical methods as square wave voltammetry and electrochemical impedance spectroscopy measurements. The designed label-free impedimetric aptasensor displayed a wide linear range from 10–11 to 10–6 mol L–1 with a sensitivity of 372 Ω per unit of log of concentration and an excellent specificity toward interfering agents such as 4,4′-dihydroxybiphenyl and bisphenol P.