The New phytologist, 2010-11, Vol.188 (3), p.774-786
2010
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- Autor(en) / Beteiligte
- Titel
- EgMYB1, an R2R3 MYB transcription factor from eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar
- Ist Teil von
- The New phytologist, 2010-11, Vol.188 (3), p.774-786
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2010
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- • The eucalyptus R2R3 transcription factor, EgMYB1 contains an active repressor motif in the regulatory domain of the predicted protein. It is preferentially expressed in differentiating xylem and is capable of repressing the transcription of two key lignin genes in vivo. • In order to investigate in planta the role of this putative transcriptional repressor of the lignin biosynthetic pathway, we overexpressed the EgMYB1 gene in Arabidopsis and poplar. • Expression of EgMYB1 produced similar phenotypes in both species, with stronger effects in transgenic Arabidopsis plants than in poplar. Vascular development was altered in overexpressors showing fewer lignified fibres (in phloem and interfascicular zones in poplar and Arabidopsis, respectively) and reduced secondary wall thickening. Klason lignin content was moderately but significantly reduced in both species. Decreased transcript accumulation was observed for genes involved in the biosynthesis of lignins, cellulose and xylan, the three main polymers of secondary cell walls. Transcriptomic profiles of transgenic poplars were reminiscent of those reported when lignin biosynthetic genes are disrupted. • Together, these results strongly suggest that EgMYB1 is a repressor of secondary wall formation and provide new opportunities to dissect the transcriptional regulation of secondary wall biosynthesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-646XeISSN: 1469-8137DOI: 10.1111/j.1469-8137.2010.03432.xTitel-ID: cdi_hal_primary_oai_HAL_hal_01565046v1
- Format
- –
- Schlagworte
- Arabidopsis, Arabidopsis - genetics, Arabidopsis - metabolism, Biochemistry, Molecular Biology, Biosynthesis, Cell Wall - metabolism, Cell walls, Cellulose, Cellulose - biosynthesis, Cellulose - genetics, Down regulation, Eucalyptus, Eucalyptus - genetics, Eucalyptus - metabolism, Fibers, Gene Expression, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene regulation, Genes, Genes, Plant, Genomics, In vivo methods and tests, Life Sciences, lignification, Lignin, Lignin - biosynthesis, Lignin - genetics, Phenotype, Phenotypes, Phloem, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Vascular Bundle - cytology, Plant Vascular Bundle - metabolism, Plants, Plants, Genetically Modified, Polymers, Poplar, Populus - genetics, Populus - metabolism, R2R3 MYB, secondary cell wall, Thickening, Transcription, transcription factor, Transcription factors, Transcription Factors - genetics, Transcription Factors - metabolism, Transgenic plants, Xylan, Xylans - biosynthesis, Xylans - genetics, Xylem