Details

Autor(en) / Beteiligte

• Le Boucher, C.
• Courant, F.
• Jeanson, S.
• Chereau, S.
• Maillard, M.-B.
• Royer, A.-L.
• Thierry, A.
• Dervilly-Pinel, G.
• Le Bizec, B.
• Lortal, S.

Titel

First mass spectrometry metabolic fingerprinting of bacterial metabolism in a model cheese

Ist Teil von

• Food chemistry, 2013-11, Vol.141 (2), p.1032-1040

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• •Mass spectrometry metabolic fingerprinting has been successfully applied to cheese.•A model cheese was inoculated with a strain of Lactococcus lactis.•Fingerprints significantly changed over time.•A large diversity of cheese metabolites was identified from MS fingerprints.•MS metabolic fingerprinting appears as a comprehensive and sensitive approach. Metabolic fingerprinting is an untargeted approach which has not yet been undertaken to investigate cheese. This study is a proof of concept, concerning the ability of mass spectrometry (MS) metabolic fingerprinting to investigate modifications induced by bacterial metabolism in cheese over time. An ultrafiltrated milk concentrate was used to manufacture model cheeses inoculated with Lactococcus lactis LD61. Metabolic fingerprints were acquired after 0, 8 and 48h from two different fractions of the metabolome: the water-soluble fraction using liquid chromatography–high resolution-MS and a volatile fraction using gas chromatography–MS. Metabolic fingerprints differed significantly over time. Forty-five metabolites were identified, including well-known cheese metabolites, such as 12 amino acids and 25 volatile metabolites, and less studied ones, such as four vitamins, uric acid, creatine and l-carnitine. These results showed the relevance of cheese MS fingerprinting to generate new findings and to detect even slight differences between two conditions.