Details

Autor(en) / Beteiligte

• Courty, Sébastien
• Luccardini, Camilla
• Bellaiche, Yohanns
• Cappello, Giovanni
• Dahan, Maxime

Titel

Tracking Individual Kinesin Motors in Living Cells Using Single Quantum-Dot Imaging

Ist Teil von

• Nano letters, 2006-07, Vol.6 (7), p.1491-1495

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.