Details

Autor(en) / Beteiligte

• Dirian, Lara
• Galant, Sonya
• Coolen, Marion
• Chen, Wenbiao
• Bedu, Sébastien
• Houart, Corinne
• Bally-Cuif, Laure
• Foucher, Isabelle

Titel

Spatial Regionalization and Heterochrony in the Formation of Adult Pallial Neural Stem Cells

Ist Teil von

• Developmental cell, 2014-07, Vol.30 (2), p.123-136

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notch invalidations, to address this issue in the zebrafish adult telencephalon. We report that the germinal zone of the adult pallium originates from two distinct subtypes of embryonic progenitors and integrates two modes of aNSC formation. Dorsomedial aNSCs derive from the amplification of actively neurogenic radial glia of the embryonic telencephalon. On the contrary, the lateral aNSC population is formed by stepwise addition at the pallial edge from a discrete neuroepithelial progenitor pool of the posterior telencephalic roof, activated at postembryonic stages and persisting lifelong. This dual origin of the pallial germinal zone allows the temporally organized building of pallial territories as a patchwork of juxtaposed compartments. [Display omitted] •Adult pallial stem cells derive from distinct embryonic progenitor subtypes•Lateral pallial stem cells are generated late during development•Cryptic boundaries linked with stem cell origin subdivide the pallial germinal zone•Large functional pallial areas are segregated from embryonic stages Dirian et al. shed light on adult neural stem cell (aNSC) embryonic origins and heterogeneity in zebrafish. They uncover dual embryonic origin for the adult pallial germinal zone. Two spatially distinct progenitor populations generate all dorsal telencephalon aNSCs and allow temporally organized building of territories as a patchwork of juxtaposed compartments.