Details

Autor(en) / Beteiligte

• Beasley, D.G.
• Alves, L.C.
• Barberet, Ph
• Bourret, S.
• Devès, G.
• Gordillo, N.
• Michelet, C.
• Le Trequesser, Q.
• Marques, A.C.
• Seznec, H.
• da Silva, R.C.

Titel

A comparison of quantitative reconstruction techniques for PIXE-tomography analysis applied to biological samples

Ist Teil von

• Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 2014-07, Vol.331, p.248-252

Ort / Verlag

Elsevier

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The tomographic reconstruction of biological specimens requires robust algorithms, able to deal with low density contrast and low element concentrations. At the IST/ITN microprobe facility new GPU-accelerated reconstruction software, JPIXET, has been developed, which can significantly increase the speed of quantitative reconstruction of Proton Induced X-ray Emission Tomography (PIXE-T) data. It has a user-friendly graphical user interface for pre-processing, data analysis and reconstruction of PIXE-T and Scanning Transmission Ion Microscopy Tomography (STIM-T). The reconstruction of PIXE-T data is performed using either an algorithm based on a GPU-accelerated version of the Maximum Likelihood Expectation Maximisation (MLEM) method or a GPU-accelerated version of the Discrete Image Space Reconstruction Algorithm (DISRA) (Sakellariou (2001) [2]). The original DISRA, its accelerated version, and the MLEM algorithm, were compared for the reconstruction of a biological sample of Caenorhabditis elegans - a small worm. This sample was analysed at the microbeam line of the AIFIRA facility of CENBG, Bordeaux. A qualitative PIXE-T reconstruction was obtained using the CENBG software package TomoRebuild (Habchi et al. (2013) [6]). The effects of pre-processing and experimental conditions on the elemental concentrations are discussed.