Details

Autor(en) / Beteiligte

• Conte, Emanuele
• Landolfi, Gianpiero
• Vincelli, Gabriele
• Stefan, Alessandra
• Hochkoeppler, Alejandro

Titel

pGOODs: new plasmids for the co-expression of proteins in

Ist Teil von

• Biotechnology letters, 2011-04, Vol.33 (9), p.1815-1821

Ort / Verlag

Springer Verlag

Erscheinungsjahr

2011

Quelle

2022 ECC(Springer)

Beschreibungen/Notizen

• Two systems for the co-expression of proteins in were designed and constructed. The first system relies on the new vector, pGOOD, which is compatible with ColE1-type plasmids and sustains efficient co-expression of soluble protein complexes. The second system is based on the pGOOD1 vector (a derivative of pGOOD), useful for the production of toxic proteins, whose synthesis can be regulated by the co-expressed LacI repressor.