Details

Autor(en) / Beteiligte

• Mannelli, I.
• Courtois, V.
• Lecaruyer, P.
• Roger, G.
• Millot, M.C.
• Goossens, M.
• Canva, M.

Titel

Surface plasmon resonance imaging (SPRI) system and real-time monitoring of DNA biochip for human genetic mutation diagnosis of DNA amplified samples

Ist Teil von

• Sensors and actuators. B, Chemical, 2006-12, Vol.119 (2), p.583-591

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Nucleic acid-based biochips represent a promising tool for gene sequence analysis, especially for mutation detection. Surface plasmon resonance imaging (SPRI) technique allows kinetic monitoring of molecular interaction, such as DNA–DNA, in real-time and without any prior labelling step. SPRI was applied to developing a DNA sensor for the detection of gene mutations accounting for human cystic fibrosis and specifically some of those localized in exon 10, with the common three-base-pair deletion ΔF508, alongside several single nucleotide polymorphisms (SNPs). The SPRI system enabled us to monitor the hybridisation kinetics of unlabelled DNA targets (short oligonucleotides or a 377 pb PCR product) to a 196 spots matrix of ssDNA probes immobilised onto a bio-functionalised surface, and to detect in real-time the mutations in a DNA fragment.