Journal of virological methods, 2006-06, Vol.134 (1), p.41-47
2006
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- Autor(en) / Beteiligte
- Titel
- A reverse transcription-nested PCR assay for HHV-6 mRNA early transcript detection after transplantation
- Ist Teil von
- Journal of virological methods, 2006-06, Vol.134 (1), p.41-47
- Ort / Verlag
- London: Elsevier B.V
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Monitoring of human herpesvirus-6 (HHV-6) reactivation is important, especially in immunocompromised patients such as transplant recipients. Reverse transcription PCR (RT-PCR) is a useful method to distinguish between latent and active infection. Here, a RT-nested PCR coupled with a colorimetric plate hybridization assay was established to detect HHV-6 types A and B U79/80 mRNAs. After confirming the reliability of the assay on HHV-6 cultures, it was applied to the detection of HHV-6 reactivation after renal (27 patients), bone marrow (14 patients) or heart (7 patients) transplantation. A total of 206 blood samples were tested from renal (137), bone marrow (58) and heart (11) transplant recipients. U79/80 mRNAs were found in 32 samples that were considered as indicative of HHV-6 reactivation: 15, 13 and 5 from kidney, bone marrow and heart transplant recipients, respectively. Finally, U79/80 mRNA detection was correlated with clinical manifestations including leucopenia, skin rash, graft rejection or dysfunction and diarrhoea.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0166-0934eISSN: 1879-0984DOI: 10.1016/j.jviromet.2005.11.015Titel-ID: cdi_hal_primary_oai_HAL_hal_00453282v1
- Format
- –
- Schlagworte
- Adult, Aged, Animals, Biological and medical sciences, Bone Marrow Transplantation, Bone Marrow Transplantation - adverse effects, Cell Line, DNA Primers, Female, Fundamental and applied biological sciences. Psychology, Genes, Viral, Genes, Viral - genetics, Heart Transplantation, Heart Transplantation - adverse effects, Herpesvirus 6, Human, Herpesvirus 6, Human - genetics, Herpesvirus 6, Human - isolation & purification, Herpesvirus 6, Human - physiology, HHV-6 reactivation, Human herpesvirus 6, Humans, Immunology, Kidney Transplantation, Kidney Transplantation - adverse effects, Life Sciences, Male, Microbiology, Middle Aged, mRNA detection, Postoperative Complications, Postoperative Complications - diagnosis, Postoperative Complications - virology, Reverse Transcriptase Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction - methods, RNA, Messenger, RNA, Messenger - genetics, RNA, Viral, RNA, Viral - genetics, Roseolovirus Infections, Roseolovirus Infections - diagnosis, Roseolovirus Infections - etiology, Roseolovirus Infections - virology, RT-PCR, Sensitivity and Specificity, Techniques used in virology, Transplant recipients, U79/80 gene, Virology, Virus Activation