Details

Autor(en) / Beteiligte

• Rajapakse, Rohan
• Mousli, Marc
• Pfaff, Alexander W.
• Uring-Lambert, Béatrice
• Marcellin, Luc
• Bronner, Christian
• Jeanblanc, Michaël
• Villard, Odile
• Letscher-Bru, Valérie
• Klein, Jean-Paul
• Candolfi, Ermanno

Titel

1,25-Dihydroxyvitamin D3 induces splenocyte apoptosis and enhances BALB/c mice sensitivity to toxoplasmosis

Ist Teil von

• The Journal of steroid biochemistry and molecular biology, 2005-07, Vol.96 (2), p.179-185

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2005

Link zum Volltext

Quelle

ScienceDirect

Beschreibungen/Notizen

• The hormonal form of Vitamin D, 1,25-dihydroxyvitamin D3, is well known for its immunosuppressive, anti-proliferative and pro-apoptotic activities. In the present work, we studied the effect of 1,25-dihydroxyvitamin D3 on Toxoplasma gondii-infected mice. We observed that 1,25-dihydroxyvitamin D3 reduces the survival rate of infected mice by up to 37% at day 10 post-infection compared to untreated infected mice ( P < 0.0001). IFN-γ and IL-12p40 levels were significantly reduced by 1,25-dihydroxyvitamin D3 in infected mice sera indicating an inhibition of Th-1-type cytokines. CD 4+ T lymphocyte and splenocyte counts were also reduced following 1,25-dihydroxyvitamin D3 treatment and a marked induction of apoptosis, accompanied with down-regulation of the anti-apoptotic proteins Bcl-2 and Bcl-X L, was observed. The above results indicate that 1,25-dihydroxyvitamin D3 induces splenocyte apoptosis and enhances host susceptibility to toxoplasmosis.