Details

Autor(en) / Beteiligte

• Nieoullon, Vincent
• Belvindrah, Richard
• Rougon, Geneviève
• Chazal, Geneviève

Titel

mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone

Ist Teil von

• Molecular and cellular neuroscience, 2005-03, Vol.28 (3), p.462-474

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2005

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• We previously showed that deletion of the cell surface molecule mCD24 resulted in an increased proliferation in adult subventricular zone (SVZ). Here, we report an increased PSA-NCAM+/TuJ1− population in the mCD24−/− in vivo SVZ as well as in vitro neurospheres. Isolated in vitro, these cells were able to generate neurospheres. Proliferation studies, using BrdU incorporation, showed an increased proliferation in P7 mCD24−/− SVZ and neurospheres. Using electron microscopy, the same cell types were identified in the in vivo SVZ as well as in vitro neurospheres from the WT and mCD24−/− mice. In mixed neurospheres, formed with WT and EGFP/KO cells (enhanced green fluorescent protein mCD24−/−), the WT environment was able to control the proliferation rate of the mCD24−/− cells, but was unable to regulate their differentiation. We concluded that mCD24 acts cell nonautonomously to regulate transit-amplifying cells proliferation and/or differentiation.