Details

Autor(en) / Beteiligte

• Touret, Nicolas
• Paroutis, Paul
• Terebiznik, Mauricio
• Harrison, Rene E.
• Trombetta, Sergio
• Pypaert, Marc
• Chow, Amy
• Jiang, Aimin
• Shaw, James
• Yip, Christopher
• Moore, Hsiao-Ping
• van der Wel, Nicole
• Houben, Diane
• Peters, Peter J.
• de Chastellier, Chantal
• Mellman, Ira
• Grinstein, Sergio

Titel

Quantitative and Dynamic Assessment of the Contribution of the ER to Phagosome Formation

Ist Teil von

• Cell, 2005-10, Vol.123 (1), p.157-170

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed ( Gagnon et al., 2002). To reconcile these seemingly disparate hypotheses, we used a combination of biochemical, fluorescence imaging, and electron microscopy techniques to quantitatively and dynamically assess the contribution of the plasmalemma and of the ER to phagosome formation and maturation. We could not verify even a transient physical continuity between the ER and the plasma membrane, nor were we able to detect a significant contribution of the ER to forming or maturing phagosomes in either macrophages or dendritic cells. Instead, our data indicate that the plasma membrane is the main constituent of nascent and newly formed phagosomes, which are progressively remodeled by fusion with endosomal and eventually lysosomal compartments as phagosomes mature into acidic, degradative organelles.