Details

Autor(en) / Beteiligte

• Aranzamendi, Maitane
• Xanthopoulou, Kyriaki
• Sánchez-Urtaza, Sandra
• Burgwinkel, Tessa
• Arazo del Pino, Rocío
• Lucaßen, Kai
• Pérez-Vázquez, M
• Oteo-Iglesias, Jesús
• Sota, Mercedes
• Marimón, Jose María
• Seifert, Harald
• Higgins, Paul G
• Gallego, Lucía

Titel

Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 IAcinetobacter baumannii/I Clone in a Tertiary Hospital in Northern Spain

Ist Teil von

• International journal of molecular sciences, 2024-02, Vol.25 (4)

Ort / Verlag

MDPI AG

Erscheinungsjahr

2024

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Infections caused by carbapenem-resistant Acinetobacter baumannii are a global threat causing a high number of fatal infections. This microorganism can also easily acquire antibiotic resistance determinants, making the treatment of infections a big challenge, and has the ability to persist in the hospital environment under a wide range of conditions. The objective of this work was to study the molecular epidemiology and genetic characteristics of two bla[sub.OXA24/40]Acinetobacter baumannii outbreaks (2009 and 2020-21) at a tertiary hospital in Northern Spain. Thirty-six isolates were investigated and genotypically screened by Whole Genome Sequencing to analyse the resistome and virulome. Isolates were resistant to carbapenems, aminoglycosides and fluoroquinolones. Multi-Locus Sequence Typing analysis identified that Outbreak 1 was mainly produced by isolates belonging to ST3[sup.Pas]/ST106[sup.Oxf] (IC3) containing bla[sub.OXA24/40], bla[sub.OXA71] and bla[sub.ADC119]. Outbreak 2 isolates were exclusively ST2[sup.Pas]/ST801[sup.Oxf] (IC2) bla[sub.OXA24/40], bla[sub.OXA66] and bla[sub.ADC30], the same genotype seen in two isolates from 2009. Virulome analysis showed that IC2 isolates contained genes for capsular polysaccharide KL32 and lipooligosacharide OCL5. A 8.9 Kb plasmid encoding the bla[sub.OXA24/40] gene was common in all isolates. The persistance over time of a virulent IC2 clone highlights the need of active surveillance to control its spread.