Details

Autor(en) / Beteiligte

• Yang, Xiaokai
• Liu, Shuai
• Wang, Zhengqiang

Titel

Ononin Alleviates Sublesional Bone Loss and Inflammation Following Spinal Cord Injury in Rats by Regulating Adenosine 5′-Monophosphate-Activated Protein Kinase Signaling Pathway

Ist Teil von

• Current topics in nutraceuticals research, 2022-11, Vol.20 (4), p.648-653

Ort / Verlag

New Century Health Publishers, LLC

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EBSCOhost Business Source Ultimate

Beschreibungen/Notizen

• Inhibition of bone loss is one of the ways to alleviate motor dysfunction caused by spinal cord injury, a lifelong and lifechanging injury. Isoflavone from legumes that include ononin, Calycosin-7-O-[beta]-Glucoside, and others have been shown to have potential for the treatment of orthopedic diseases. While there are potentials for ononin to improve the motor function of spinal cord injury, studies on the subject are lacking. To this end, we examined the ameliorative role of ononin in an animal model of spinal cord injury. Our results show the ononin reduced inflammatory response, decreased femoral bone loss, and promoted osteoblast formation in rats with spinal cord injury through promotion of adenosine 5"-monophosphate-activated protein kinase signaling pathway. Keywords: AMPK pathway, Basso-beattie-bresnahan, Inflammatory response, Ononin, Spinal cord injury Abbreviations Used: Alkaline phosphatase, ALP; Adenosine 5'-monophosphate-activated protein kinase, AMPK; Basso-beattiebresnahan, BBB; Bone mineral density, BMD; C[a.sup.2+]/calmodulin-dependent protein kinase, CAMK; Collagen Type 1 Alpha 1 chain, COL1A1; Enhanced chemiluminescence, ECL; Extracellular signal-regulated kinase, ERK; Glycogen synthase kinase, GSK; Hematoxylin-eosin, HE; Horse radish peroxidase, HRP; lnterleukin-1[beta], IL-1[beta]; lnterleukin-6, IL-6; Mitogen-activated protein kinase, MEK; Mammalian target of rapamycin, mTOR; Nuclear factor-kappa B, NF-KB; Ononin, ON; Osteopontin, OPN; A REL-associated protein involved in NF-KB heterodimer formation, nuclear translocation, and activation, p65; Phosphatidylinositol-specific phospholipase Cy2, PLCy2; p-nitrophenyl phosphate, pNPP; Polyvinylidene difluoride, PVDF; Runt-related transcription factor 2, RUNX2; Spinal cord injury, SCI; Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis, SDS-PAGE; Standard error of mean, SEM; Tris-buffered saline, TBST; Tumor necrosis factor-a, TNF-a; A portmanteau of int and Wg and stands for "Wingless-related integration site," Wnt Corresponding Author: Dr. Zhengqiang Wang, Department of Orthopedic Surgery, Taikang Tongji (Wuhan) Hospital, No. 322 Sixin North Road, Hanyang District, Wuhan City, Hubei Province, China; E-mail: zq_wang82@163.com