Details

Autor(en) / Beteiligte

• Touloudi, Antonia
• McGiven, John
• Cawthraw, Shaun
• Valiakos, George
• Kostoulas, Polychronis
• Duncombe, Lucy
• Gortázar, Christian
• Boadella, Mariana
• Sofia, Marina
• Athanasakopoulou, Zoi
• Chatzopoulos, Dimitris C
• Spyrou, Vassiliki
• Petrovska, Liljana
• Billinis, Charalambos

Titel

Development of a Multiplex Bead Assay to Detect Serological Responses to IBrucella/I Species in Domestic Pigs and Wild Boar with the Potential to Overcome Cross-Reactivity with IYersinia enterocolitica/I O:9

Ist Teil von

• Microorganisms (Basel), 2022-07, Vol.10 (7)

Ort / Verlag

MDPI AG

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The aim of this study was to develop a multiplex bead assay using a Brucella rLPS antigen, a Brucella suis smooth antigen, and a Yersinia enterocolitica O:9 antigen that not only discriminates Brucella-infected from Brucella-uninfected pigs and wild boar, but also overcomes the cross reactivity with Y. enterocolitica O:9. Sera from 126 domestic pigs were tested: 29 pigs were Brucella infected, 80 were non-infected and 17 were confirmed to be false positive serological reactors (FPSR). Sera from 49 wild boar were tested: 18 were positive and 31 were negative. Using the rLPS antigen, 26/29 Brucella-infected domestic pigs and 15/18 seropositive wild boar were positive, while 75/80 non-Brucella infected domestic pigs, all FPSR, and all seronegative wild boar were negative. Using the smooth B. suis 1330 antigen, all Brucella-infected domestic pigs, 9/17 FPSR and all seropositive wild boar were positive, while all non-infected pigs and 30/31 seronegative wild boar were negative. The ratio of the readouts from the smooth B. suis antigen and Y. enterocolitica O:9 antigen enabled discriminating all Brucella infected individuals from the FPSR domestic pigs. These results demonstrate the potential of this assay for use in the surveillance of brucellosis, overcoming the cross-reactivity with Y. enterocolitica.