Details

Autor(en) / Beteiligte

• Wang, Yuanhong
• Zhang, Fang
• Yu, Chunmei
• Tu, Yifeng
• Miao, Peng
• Gu, Haiying

Titel

Enhanced and tunable oxygen carrier and amperometric sensor based on a glassy carbon electrode assembly of a hemoglobin-chitosan-Fe.sub.3O.sub.4 composite

Ist Teil von

• Mikrochimica acta (1966), 2017-05, Vol.184 (5), p.1437

Ort / Verlag

Springer

Erscheinungsjahr

2017

Quelle

SpringerLink

Beschreibungen/Notizen

• The authors have prepared organized assemblies of a hemoglobin-chitosan(CS)@Fe.sub.3O.sub.4 composite on glassy carbon electrodes (GCEs) via three strategies with the aim of preparing tunable Hb-coated GCEs with good stability and long-term oxygen storage capability. The formation and morphology of the Hb-CS@Fe.sub.3O.sub.4 composite was characterized by scanning electrochemical microscopy, XRD and UV-vis spectroscopy. It is shown that Hb is fully integrated into the CS@Fe.sub.3O.sub.4 and can be manipulated by a magnetic field whilst maintaining its biological activity. In the absence of oxygen, a surface-controlled electrode process occurs with an interfacial electron transfer rate (k.sub.s) of 2.14 s.sup.-1. The modified GCE also has a favorable oxygen storage lifetime (almost 6 h). One Hb-CS@Fe.sub.3O.sub.4 film on the electrode displays particularly good electrocatalytic reduction activity towards oxygen. The linear range for detection of O.sub.2 is 1.2 x 10.sup.-7 ~ 2.0 x 10.sup.-4 mol L.sup.-1 with a detection limit of 4.0 x 10.sup.-8 mol L.sup.-1. In our opinion, this method has great potential in terms of enhanced oxygen storage capability of Hb, which can be applied in special situations such as space operations, down hole mining, mountaineering and diving.