Details

Autor(en) / Beteiligte

• Chaves, Barbara Aparecida
• Junior, Ademir Bentes Vieira
• Silveira, Karine Renata Dias
• Paz, Andreia Da Costa
• Vaz, Evelyn Beatriz Da Costa
• Araujo, Raphaela Guedes Pereira
• Rodrigues, Nilton Barnabe
• Campolina, Thais Bonifacio
• Orfano, Alessandra Da Silva
• Nacif-Pimenta, Rafael
• Villegas, Luis Eduardo Ma
• Melo, Fabricio Freire De
• Silva, Breno De Mello
• Monteiro, Wuelton Marcelo
• Guerra, Maria Das Gracas Vale Barbosa
• Lacerda, Marcus Vinicius Guimaraes De
• Norris, Douglas Eric
• Secundino, Nagila Francinete Costa
• Pimenta, Paulo Filemon Paolucci

Titel

Vertical Transmission of Zika Virus Delays Egg Hatching and Larval Development of Progeny

Ist Teil von

• Journal of medical entomology, 2019-11, Vol.56 (6), p.1739

Ort / Verlag

Oxford University Press

Erscheinungsjahr

2019

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third-fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch.The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. Amazonian Ae. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector. Key words: Zika virus, vertical transmission, Aedes aegypti, fitness cost