Details

Autor(en) / Beteiligte

• Muller, Paula
• Voronina, Natalia
• Hausburg, Frauke
• Lux, Cornelia A
• Wiekhorst, Frank
• Steinhoff, Gustav
• David, Robert

Titel

Magnet-Bead Based MicroRNA Delivery System to Modify [CD133.sup.+] Stem Cells

Ist Teil von

• Stem cells international, 2016-01

Ort / Verlag

John Wiley & Sons, Inc

Erscheinungsjahr

2016

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Aim. [CD133.sup.+] stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended to develop a nonviral system for appropriate cell engineering. Materials and Methods. Modification of human [CD133.sup.+] stem cells with magnetic polyplexes carrying microRNA was studied in terms of efficiency, safety, and targeting potential. Results. High microRNA uptake rates (~80--90%) were achieved without affecting [CD133.sup.+] stem cell properties. Modified cells can be magnetically guided. Conclusion. We developed a safe and efficient protocol for [CD133.sup.+] stem cell modification. Our work may become a basis to improve stem cell therapeutical effects as well as their monitoring with magnetic resonance imaging.