Details

Autor(en) / Beteiligte

• D'Amico, Gabriela
• Robinson, Stephen D
• Germain, Mitchel
• Reynolds, Louise E
• Thomas, Gareth J
• Elia, George
• Saunders, Garry
• Fruttiger, Marcus
• Tybulewicz, Victor
• Mavria, Georgia
• Hodivala-Dilke, Kairbaan M

Titel

Endothelial-Rac1 Is Not Required for Tumor Angiogenesis unless [alpha]v[beta]3-Integrin Is Absent

Ist Teil von

• PloS one, 2010-03, Vol.5 (3), p.e9766

Ort / Verlag

Public Library of Science

Erscheinungsjahr

2010

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Endothelial cell migration is an essential aspect of tumor angiogenesis. Rac1 activity is needed for cell migration in vitro implying a requirement for this molecule in angiogenesis in vivo. However, a precise role for Rac1 in tumor angiogenesis has never been addressed. Here we show that depletion of endothelial Rac1 expression in adult mice, unexpectedly, has no effect on tumor growth or tumor angiogenesis. In addition, repression of Rac1 expression does not inhibit VEGF-mediated angiogenesis in vivo or ex vivo, nor does it affect chemotactic migratory responses to VEGF in 3-dimensions. In contrast, the requirement for Rac1 in tumor growth and angiogenesis becomes important when endothelial [beta]3-integrin levels are reduced or absent: the enhanced tumor growth, tumor angiogenesis and VEGF-mediated responses in [beta]3-null mice are all Rac1-dependent. These data indicate that in the presence of [alpha]v[beta]3-integrin Rac1 is not required for tumor angiogenesis.