Details

Autor(en) / Beteiligte

• Valver de Malaver, Claudia Lorena
• Colmenares Dulcey, Ana Julia
• Isaza Martínez, José Hipólito

Titel

Comparison of DPPH free radical scavenging, ferric reducing antioxidant power

Ist Teil von

• Revista de ciencias, 2015-12, Vol.19 (2), p.117

Ort / Verlag

Universidad del Valle

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Searching for new antioxidants used in pharmaceuticals, cosmetics or agrochemicals have increased today. Many phenolic compounds have been reported as promising for this goal. Melastomataceae is rich in these compounds. Consequently, in this research the antioxidant power for Meriania nobilis and M. hemandoi (Melastomataceae) was compared. Both species were extracted in acetone 70%. The latest was liquid-liquid partitioned in ethyl acetate (Mn or Mh 2.1), n-butanol (Mn or Mh 2.2) and aqueous fractions (Mn or Mh 2.3). Antioxidant activity was measured by DPPH free radical scavenging method ([FRS.sub.50], mg.[L.sup.-1]), ferric reducing antioxidant power assay (FRAP, mg FeS[O.sub.4].7[H.sub.2]O [(100 g).sup.-1]ES) and total phenolic content (TPC, mg AG [g.sup.-1]ES) by Folin- Ciocalteu. The present study revealed that Mh 2.1 and Mh 2.2 from M. hernandoi have a strong antioxidant power [FRS.sub.50] = 5.71 ± 0.085, FRAP = 53 ± 1.5 and TPC = 224 ± 7.1 and [FRS.sub.50] = 4.22 ± 0.001, FRAP = 65 mg [+ or -] 1.5, TPC = 240 ± 8.6 respectively, as compared with positive control, quercetin DPPH [FRS.sub.50] = 4.20 ± 0.410, FRAP = 61 [+ or -] 1.3. M. nobilis exhibited poor antioxidant power [FRS.sub.50] = 286 [+ or -] 1.5, FRAP = 2.01 ± 0.079 and TPC = 26 ± 1.5. This paper showed M. hernandoi as good source of antioxidant compounds with potential usage as cosmetic, pharmaceutical or agrochemical ingredients. Keywords: Antioxidant power, DPPH free radical scavenging method (FRS), ferric reducing antioxidant power assay (FRAP), Estimation of total phenolic content (TPC), Meriania nobilis, Meriania hernandoi.