Details

Autor(en) / Beteiligte

• Xu, Jinhua
• Prosperi, Jenifer R
• Choudhury, Noura
• Olopade, Olufunmilayo I
• Goss, Kathleen H

Titel

[beta]-Catenin Is Required for the Tumorigenic Behavior of Triple-Negative Breast Cancer Cells

Ist Teil von

• PloS one, 2015-02, Vol.10 (2), p.e0117097

Ort / Verlag

Public Library of Science

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Our previous data illustrated that activation of the canonical Wnt signaling pathway was enriched in triple-negative breast cancer and associated with reduced overall survival in all patients. To determine whether Wnt signaling may be a promising therapeutic target for triple-negative breast cancer, we investigated whether [beta]-catenin was necessary for tumorigenic behaviors in vivo and in vitro. [beta]-catenin expression level was significantly reduced in two human triple-negative breast cancer cell lines, MDA-MB-231 and HCC38, using lentiviral delivery of [beta]-catenin-specific small hairpin RNAs (shRNAs). Upon implantation of the cells in the mammary fat pad of immunocompromised mice, we found that [beta]-catenin shRNA HCC38 cells formed markedly smaller tumors than control cells and grew much more slowly. In in vitro assays, [beta]-catenin silencing significantly reduced the percentage of Aldefluor-positive cells, a read-out of the stem-like cell population, as well as the expression of stem cell-related target genes including Bmi-1 and c-Myc. [beta]-catenin-knockdown cells were also significantly impaired in their ability to migrate in wound-filling assays and form anchorage-independent colonies in soft agar. [beta]-catenin-knockdown cells were more sensitive to chemotherapeutic agents doxorubicin and cisplatin. Collectively, these data suggest that [beta]-catenin is required for triple-negative breast cancer development by controlling numerous tumor-associated properties, such as migration, stemness, anchorage-independent growth and chemosensitivity.