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Yersinia pseudotuberculosis disrupts intestinal barrier integrity through hematopoietic TLR-2 signaling
Ist Teil von
The Journal of clinical investigation, 2012-06, Vol.122 (6), p.2239
Ort / Verlag
American Society for Clinical Investigation
Erscheinungsjahr
2012
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
Intestinal barrier function requires intricate cooperation between intestinal epithelial cells and immune cells. Enteropathogens are able to invade the intestinal lymphoid tissue known as Peyer's patches (PPs) and dis-rupt the integrity of the intestinal barrier. However, the underlying molecular mechanisms of this process are poorly understood. In mice infected with Yersinia pseudotuberculosis, we found that PP barrier dysfunc-tion is dependent on the Yersinia virulence plasmid and the expression of TLR-2 by hematopoietic cells, but not by intestinal epithelial cells. Upon TLR-2 stimulation, Y. pseudotuberculosis-infected monocytes activated caspase-1 and produced IL-1β. In turn, IL-1β increased NF-[k]B and myosin light chain kinase activation in intestinal epithelial cells, thus disrupting the intestinal barrier by opening the tight junctions. Therefore, Y. pseudotuberculosis subverts intestinal barrier function by altering the interplay between immune and epi-thelial cells during infection.