Details

Autor(en) / Beteiligte

• Wang, Qi
• Qiao, Mengwei
• Song, Jinzhu

Titel

Characterization of Two Na[sup.+]/H[sup.+] Antiporters from INatronorubrum daqingense/I

Ist Teil von

• International journal of molecular sciences, 2023-06, Vol.24 (13)

Ort / Verlag

MDPI AG

Erscheinungsjahr

2023

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The Na[sup.+]/H[sup.+] antiporter NhaC family protein is a kind of Na[sup.+]/H[sup.+] exchanger from the ion transporter (IT) superfamily, which has mainly been identified in the halophilic bacteria of Bacillus. However, little is known about the Na[sup.+]/H[sup.+] antiporter NhaC family of proteins in the extremely halophilic archaea. In this study, two Na[sup.+]/H[sup.+] antiporter genes, nhaC1 and nhaC2, were screened from the genome of Natronorubrum daqingense based on the gene library and complementation of salt-sensitive Escherichia coli KNabc. A clone vector pUC18 containing nhaC1 or nhaC2 could make KNabc tolerate 0.6 M/0.7 M NaCl or 30 mM/40 mM LiCl and a pH of up to 8.5/9.5, respectively. Functional analysis shows that the Na[sup.+](K[sup.+], Li[sup.+])/H[sup.+] antiport activities of NhaC1 and NhaC2 are both pH-dependent in the range of pH 7.0–10.0, and the optimal pH is 9.5. Phylogenetic analysis shows that both NhaC1 and NhaC2 belong to the Na[sup.+]/H[sup.+] antiporter NhaC family of proteins and are significantly distant from the identified NhaC proteins from Bacillus. In summary, we have identified two Na[sup.+](K[sup.+], Li[sup.+])/H[sup.+] antiporters from N. daqingense.