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SDS-PAGE electrophoretic pattern of pork loin enhanced with non-meat proteins/ Perfil eletroforetico de lombo suino adicionado de proteinas nao carneas
Ist Teil von
Ciência rural, 2010-02, Vol.40 (2), p.434
Ort / Verlag
Universidade Federal de Santa Maria
Erscheinungsjahr
2010
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Brazilian meat regulations allow the use of nonmeat extenders in certain meat products. Thus, the need for controlling the use of these ingredients with proper analytical methods is evident, in order to avoid illegal addition. In the present research, SDS-PAGE electrophoresis has been applied to pork enhanced with 1.5% of soy protein isolate and whey protein concentrate, through two different methods of protein extraction (urea 6M and Tris-HCl-SDS-mercaptoethanol), in the presence of positive and negative controls. Electrophoresis has revealed typical profiles, for whole-muscle and non-meat proteins, showing to be adequate to detect the addition of whey proteins in meat products. However, the soy proteins could not be detected, due to the overlapping of bands with meat proteins. Key words: meat products, frauds, electrophoresis, non-meat ingredients. A legislacao brasileira permite a adicao intencional de proteinas nao carneas em alguns produtos carneos; entretanto, ha poucas tecnicas adaptadas e proprias para controle desses ingredientes. Na presente pesquisa, empregou-se a tecnica de eletroforese em gel de poliacrilamida na presenca de dodecil-sulfato de sodio (SDS-PAGE) para comparar amostras de carne suina (lombo), adicionadas de 1,5% de proteinas nao carneas (proteina isolada de soja e concentrado proteico de soro de leite). Mediante a padronizacao de dois protocolos distintos de extracao (ureia 6M e Tris-HCl-SDS-mercaptoetanol) e a utilizacao de controles positivos e negativos, obtiveram-se perfis eletroforeticos tipicos do musculo e das proteinas utilizadas. Ambas as extracoes utilizadas foram adequadas para separacao das proteinas do soro de leite das proteinas da carne, mas a visualizacao dos marcadores da soja nao foi possivel em razao da sobreposicao de bandas. Palavras-chave: carnes, fraudes, eletroforese, proteinas nao carneas.