Details

Autor(en) / Beteiligte

• ZVONIC, Sanjin
• PTITSYN, Andrey A
• CONRAD, Steven A
• SCOTT, L. Keith
• FLOYD, Z. Elizabeth
• KILROY, Gail
• XIYING WU
• GOH, Brian C
• MYNATT, Randall L
• GIMBLE, Jeffrey M

Titel

Characterization of Peripheral Circadian Clocks in Adipose Tissues

Ist Teil von

• Diabetes (New York, N.Y.), 2006-04, Vol.55 (4), p.962-970

Ort / Verlag

Alexandria, VA: American Diabetes Association

Erscheinungsjahr

2006

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Characterization of Peripheral Circadian Clocks in Adipose Tissues Sanjin Zvonic 1 , Andrey A. Ptitsyn 2 , Steven A. Conrad 3 , L. Keith Scott 3 , Z. Elizabeth Floyd 1 , Gail Kilroy 1 , Xiying Wu 1 , Brian C. Goh 1 , Randall L. Mynatt 1 and Jeffrey M. Gimble 1 1 Stem Cell Laboratory, Louisiana State University Pennington Biomedical Research Center, Baton Rouge, Louisiana 2 Experimental Obesity Laboratory, Louisiana State University Pennington Biomedical Research Center, Baton Rouge, Louisiana 3 Departments of Bioinformatics and Computational Biology, Medicine, and Emergency Medicine, Louisiana State University Health Sciences Center, Shreveport, Louisiana Address correspondence and reprint requests to Jeffrey M. Gimble, 6400 Perkins Rd., Baton Rouge, LA 70808. E-mail: GimbleJM{at}pbrc.edu Abstract First described in the suprachiasmatic nucleus, circadian clocks have since been found in several peripheral tissues. Although obesity has been associated with dysregulated circadian expression profiles of leptin, adiponectin, and other fat-derived cytokines, there have been no comprehensive analyses of the circadian clock machinery in adipose depots. In this study, we show robust and coordinated expression of circadian oscillator genes ( Npas2 , Bmal1 , Per1-3 , and Cry1-2 ) and clock-controlled downstream genes ( Rev-erb α, Rev-erbβ , Dbp , E4bp4 , Stra13 , and Id2 ) in murine brown, inguinal, and epididymal (BAT, iWAT, and eWAT) adipose tissues. These results correlated with respective gene expression in liver and the serum markers of circadian function. Through Affymetrix microarray analysis, we identified 650 genes that shared circadian expression profiles in BAT, iWAT, and liver. Furthermore, we have demonstrated that temporally restricted feeding causes a coordinated phase-shift in circadian expression of the major oscillator genes and their downstream targets in adipose tissues. The presence of circadian oscillator genes in fat has significant metabolic implications, and their characterization may have potential therapeutic relevance with respect to the pathogenesis and treatment of diseases such as obesity, type 2 diabetes, and the metabolic syndrome. BAT, brown adipose tissue bHLH-PAS, basic helix-loop-helix/Per-Arnt-Simpleminded DBP, albumin d-element–binding protein eWAT, epididymal adipose tissue iWAT, inguinal adipose tissue PAI-1, plasminogen activator inhibitor-1 SCN, suprachiasmatic nucleus Footnotes X.W. holds stock in Eli Lilly and Pfizer. J.M.G. has served on advisory panels for Cognate Therapeutics, Artecel Sciences, Vesta Therapeutics, Vet-Stem, and Zen-Bio; holds stock in Eli Lilly and Pfizer; has received honoraria from Pfizer; and has been a paid consultant for Cognate Therapeutics, Artecel Sciences, and Anterogen. Additional information for this article can be found in an online appendix at http://diabetes.diabetesjournals.org . The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Accepted December 20, 2005. Received July 8, 2005. DIABETES