Details

Autor(en) / Beteiligte

• Arancibia, Sergio
• Barrientos, Andrea
• Torrejón, Javiera
• Escobar, Alejandro
• Beltrán, Caroll J

Titel

Copper oxide nanoparticles recruit macrophages and modulate nitric oxide, proinflammatory cytokines and PGE

Ist Teil von

• Nanomedicine (London, England), 2016-05, Vol.11 (10), p.1237-1251

Ort / Verlag

Future Medicine Ltd

Erscheinungsjahr

2016

Beschreibungen/Notizen

• : In the present study, we examine the effects of copper oxide nanoparticles (CuNP) on macrophage immune response and the signaling pathways involved. A peritonitis model was used to determine immune cells recruitment, while primary macrophages were used as an model for the cellular and molecular analysis. , CuNP induce significant macrophages recruitment to the site of injection. , in LPS-stimulated primary macrophages, the co-treatment with CuNP inhibited the production of NO in a dose-dependent manner. The mechanism underlying NO and proinflammatory cytokines inhibition was associated with an increased arginase activity. Macrophage stimulation with CuNP did not provoke any cytokine secretion; however, arginase inhibition promoted TNFα and MIP-1β production. In addition, CuNP induced the expression of COX-2 and the production of PGE through arginase activation. Our results demonstrate that CuNP activate arginase and suppress macrophage innate immune response.