Details

Autor(en) / Beteiligte

• Staigmiller, R.B. (USDA, ARS, Fort Keogh Livestock and Range Research Laboratory, Miles City, MT)
• Bellows, R.A
• Anderson, G.B
• Seidel, G.E. Jr
• Foote, W.D
• Menino, A.R. Jr
• Wright, R.W. Jr

Titel

Superovulation of cattle with equine pituitary extract and porcine FSH

Ist Teil von

• Theriogenology, 1992-05, Vol.37 (5)

Erscheinungsjahr

1992

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Superovulation has been practiced in cattle for more than 50 years but the results have been highly variable. Scientists at six locations compared a horse pituitary extract (RAP) with a single batch of porcine FSH (pFSH) to determine the efficacy of these hormones to induce superovulation and to test for variability in the superovulatory response. Acetone-dried equine pituitaries were suspended in 40% ethanol containing 6% ammonium acetate, and the supernatant was mixed with 2.5 volumes of cold ethanol. The resulting precipitate was washed with cold ether and dried. Total doses of 18 mg of HAP and 36 mg of pFSH were injected intramuscularly (i.m.) over 4 days, two injections per day, and prostaglandin (PGF2 alpha; 25 mg, i.m,) was administered on Day 3. Injections were begun on Days 6 to 13 of the estrous cycle. The overall ovulation rates (mean +/- SEM) for HAP and FSH were 8.8 +/- 0.7 and 15.1 +/- 1.0, respectively (n = 231; P 0.01). Location interacted (P 0.01) with the type of gonadotropin for the ovulation rate. When expressed as a proportion of the number of corpora lutea, the total number of embryos recovered was greater (P