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Journal of Bacteriology, 1989-01, Vol.171 (1), p.230-237
1989
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Autor(en) / Beteiligte
Titel
The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis
Ist Teil von
  • Journal of Bacteriology, 1989-01, Vol.171 (1), p.230-237
Ort / Verlag
United States: American Society for Microbiology
Erscheinungsjahr
1989
Quelle
MEDLINE
Beschreibungen/Notizen
  • The REV1 gene of Saccharomyces cerevisiae is required for normal induction of mutations by physical and chemical agents. We have determined the sequence of a 3,485-base-pair segment of DNA that complements the rev1-1 mutant. Gene disruption was used to confirm that this DNA contained the REV1 gene. The sequenced segment contains a single long open reading frame, which can encode a polypeptide of 985 amino acid residues. The REV1 transcript is 3.1 kilobase pairs in length. Frameshift mutations introduced into the open reading frame yielded a Rev- phenotype. A base substitution, encoding Gly-193 to Arg-193, was found in this open reading frame in rev1-1. Deletion mutants, lacking segements of the 5' region of REV1, had intermediate mutability relative to REV1 and rev1-1; a complete deletion exhibited lower mutability than rev1-1. REV1 is not an essential gene. An in-frame fusion of the 5' end of the REV1 open reading frame to the lacZ gene produced beta-galactosidase activity constitutively. The predicted REV1 protein is hydrophilic, with a predicted pI of 9.82. No homologies to RAD1, RAD2, RAD3, RAD7, or RAD10 proteins were noted. A 152-residue internal segment displayed 25% identity with UMUC protein

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