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2'4'-Dihidroxy-5'-(1?-dimethylallyl)-6-prenylpinocembrin was obtained from roots of Dalea elegans. Antimicrobial assays have been proved its antibacterial activity against oxacillin-sensitive and resistant Staphylococcus aureus, and antifungal activity against Candida albicans, some strains of Candida glabrata, Candida krusei, Cryptococcus neoformans and a strain of Trychophyton mentagrophytes, which were clinically isolated multiresistant strains.